Sorting and analysis of hematopoietic stem cells and myeloid lineage-committed progenitors using flow cytometry
- VernacularTitle:采用多功能流式细胞术分析分选造血干细胞和髓系定向分化祖细胞
- Author:
Wei CUI
;
Xiaodong XU
;
Yonggang XU
;
Xuan WANG
- Publication Type:Journal Article
- Keywords:
Flow cytometry;
Soring;
Hematopoietic stem cells;
Myeloid;
Progenitors
- From:
Chinese Journal of Laboratory Medicine
2008;31(5):494-498
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the experimental protocol for purification and analysis of hematopoietic stem cells(HSC)and myeloid lineage-committed progenitors.Methods According to differentiation antigen expression pattern on hematopoietic stem cells(HSC) and progenitors during hematopoietic development,HSC and progenitors from bone marrow of 14 healthy mice were analyzed and sorted by magnetic nanoparticles and 4-color or 6-color flow cytometry using multiple antibody panels.Sorted HSC and progenitors were further tested by methylcellulose colony forming unit(CFU)and serial replatingassays.Results The expression of hematopoietic progenitor cells(HPC)was 10-fold higher expression than that of HSC.However,replating activity of common myeloid rogenitors(CMP)was only half of that of HSC.And there was almost 120 replating activity observed in granulocyte/macrophage lineage-restricted progenitors(GMP)and megakaryocyte/erythroeyte lineage-restricted progenitors(MEP).Conclusion Multiparametric flow cytometry could be used to isolate and count HSC and myeloid lineage-committed progenitors accurately.
