Effect of inhibition of hPOT1 by RNA interference on gene expression of TRF1, TRF2 and Tankyrase1 in human gastric cancer cell BGC823
- VernacularTitle:hPOT1 RNA干扰对人胃癌BGC823细胞TRF1、TRF2和Tankyrase1表达的影响
- Author:
Xiaoyan NING
;
Dianchun FANG
;
Yicheng LI
;
Liping GUO
;
Jun TIE
;
Shiming YANG
;
Rongquan WANG
;
Guiyong PENG
;
Wensheng CHEN
- Publication Type:Journal Article
- Keywords:
Telomere;
RNA interference;
Transcription;
Gene expression
- From:
Chinese Journal of Digestive Endoscopy
2008;25(6):309-312
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of silence of human protection of telomeres 1 (hPOT1), which was induced by RNA interference, on expression of telomeric repeat factor 1 (TRF1), telomeric repeat factor 2 (TRF2) and Tankyrase 1 in human gastric cancer cell BGC823. Methods The ex-pression of TRF1 ,TRF2 and Tankyrasel at mRNA level were determined by semi-quantitative RT-PCR. Re-sults Significant increase in expression of TRFI, marked decrease of TRF2 and Tankyrase1 at mRNA level were observed in cells of hPOT1 siRNA. Conclusion The significant increase in expression of TRF1 and the marked decease in TRF2 and Tnakyrasel at mRNA level after the inhibited expression of hPOT1 in human gastric cancer cell BGC823 indicate that hPOTI is highly correlated with the expressions of other three te-lomere-specific binding proteins.
