Expression and function of CIP4 in renal interstitial fibrosis
10.3760/cma.j.issn.1001-7097.2010.06.010
- VernacularTitle:CIP4在肾间质纤维化中的表达及作用
- Author:
Shoujun BAI
;
Yamin ZHANG
;
Qiaodan ZHOU
;
Rui ZENG
;
Caixia LI
;
Guangchang PEI
;
Chuou XU
;
Shuwang GE
;
Huan ZHOU
;
Gang XU
;
Xiaocheng LIU
- Publication Type:Journal Article
- Keywords:
Fibrosis;
Kidney tubules;
Epithelial cells;
Beta-catenin;
Cdc42 interacting protein-4
- From:
Chinese Journal of Nephrology
2010;26(6):453-459
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression and localization of CIP4 (Cdc42 interacting protein-4) in the renal fibrosis and the effect of CIP4 on the expression of E-cadherin,vimentin and β-catenin tyrosine phosphorylation. Methods In vitro, the human tubular epithelial cells (HK-2 cell line) were cultured with 10 μg / L TGF-β1 for 72 h. The protein expressions of CIP4, E-cadherin, vimentin and β-catenin tyrosine phosphorylation were measured by Western blotting; the expression of CIP4 mRNA was detected by RT-PCR. The intracellular distribution of CIP4 was observe by confocal microscope. In vivo, Masson staining was used to evaluate the level of renal fibrosis; the expression and distribution of CIP4 in renal tissue were detected by immunohistochemistry. HK-2 cells were transfected with pcDNA3. 1-CIP via lipofectamine 2000. The expressions of E-cadherin, vimentin and β-catenin tyrosine phosphorylation level in the transfected cells were detected by Western blotting. Results The expressions of CIP4 mRNA and protein were up-regulated in renal tubular EMT cells. Most of CIP4 protein localized in cell membrane, and some was in cytoplasm. After stimulation by TGF-β1, the expression of CIP4 protein both in cytoplasm and nucleus was greatly increased (P ＜0.05),especially in cytoplasm. In vivo, CIP4 was expressed in renal tubular epithelia, but little expressed in glomeruli. In renal from 5/6 nephrectomized rats, CIP4 expression was significantly increased. In the CIP4 transfectants, the expression of CIP4, vimentin and β-catenin tyrosine phosphorylation level were up-regulated (P ＜0.05), but E-cadherin expression was suppressed (P ＜0.05).Conclusion The overexpression of CIP4 is likely to take part in the epithelial-to-mesenchymal transition process, thereby promoting the renal fibrosis.
