Molecular characteristic analysis of E gene in genotype 3 Japanese encephalitis virus in China
10.3760/cma.j.issn.0254-5101.2010.05.001
- VernacularTitle:中国基因3型乙型脑炎病毒E基因分子特征
- Author:
Huanyu WANG
;
Guodong LIANG
- Publication Type:Journal Article
- Keywords:
Japanese encephalitis virus;
Homology;
Molecular character
- From:
Chinese Journal of Microbiology and Immunology
2010;30(5):387-391
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the molecular characteristics on nucleotide and amino acid in E gene through the comparative analysis of differences between genotype 3 Japanese encephalitis virus(JEV)in China and the live attenuated vaccine(SA14-14-2).Methods Obtained the E gene nucleotide sequence of Japanese encephalitis virus from GenBank.Multiple alignment and amino acid(AA)analysis were carried out by Clustal X(1.81),DNAStar and GENEDOC(3.2).According to the ribbon diagram of the E protein structure based on the model of the soluble fragment of tick-borne encephalitis virus(TBEV)as a template for Japanese encephalitis virus E protein amino acid sequence analysis.Results Compared different regions and different host genotype 3 JEV with SA14-14-2 the nucleotide sequence homology was,up 96%and 95% and the amino acid homology was up 95%and 94%,respectively.The nucleotide and amino acid homology was higher among the same area and the same host.Ten common amino acid variations and five special variations(E160 in domain Ⅰ,E123 and E227 in domain Ⅱ,E441 and E487 in non-structural domain)were found between genotype 3 JEV and SA14-14-2.Conclusion The homology in E gene was higher between genotype 3 JEV in China and SA14-14-2.Five special amino acid differences were found in genotype 3 JEV.The existing live attenuated vaccine strain is theoretically possible to protect genotype 3 JEV in China.
