Detection on the pandemic( H1N1 ) 2009 virus of an influenza epidemic situation and study on the characteristic of HA and NA gene

VernacularTitle:一起甲型H1N1流感疫情病原检测及其HA与NA基因特性研究
Author: Xia LEI ; Yan HAI ; Xin LI ; Weidong GUO
Publication Type:Journal Article
Keywords: Pandemic(H1N1 ) 2009; Real-time RT-PCR; HA gene; NA gene
From: Chinese Journal of Microbiology and Immunology 2010;30(7):626-630
CountryChina
Language:Chinese
Abstract: Objective To identify the pathogen of an influenza epidemic situation and analyze the genetic characteristic of hemagglutinin( HA ) gene and neuraminidase(NA) gene of this pathogen. Methods Real-time RT-PCR was used to dectect nucleic acid of the pandemic( H1N1 ) 2009 virus from oropharyngeal swabs of initial influenza-like illness in epidemic. The viruses were was inoculated and isolated with embryonated eggs. And the HA gene and NA gene were sequenced to analyze their characteristic. Results The influenza epidemic situation was caused by the pandemic( H1N1 ) 2009 virus. The HA and NA sequences data showed that the virus had the high homology with reference virus, and the NA sequences had not the H274Y mutation. Conclusion In this study, the pandemic( H1N1 ) 2009 virus were similar with the vaccine-like virus and the isolated virus of China, and sensitive to oseltamivir.