Serial passage of clade C SHIV-XJ02170 in Chinese origin Rhesus macaques

VernacularTitle:C亚型SHIV-XJ02170在中国恒河猴体内传代研究
Author: Qiang LIU ; Yue LI ; Guibo YANG ; Qiang WEI ; Chuan QIN ; Yiming SHAO
Publication Type:Journal Article
Keywords: SHIV; Passage; Rhesus macaques; Model
From: Chinese Journal of Microbiology and Immunology 2010;30(6):501-505
CountryChina
Language:Chinese
Abstract: Objective To analyze the virologic and immunologic properties during SHIV-XJ02170passage in vivo and construct the clade C SHIV/Chinese origin Rhesus macaques AIDS model . Methods SHIV-XJ02170 cell-free virus tranfected in 293T was adapted by serial passage in nine Chinese-origin Rhesus macaques. CD4/CD8 ratio was detected by flow cytometry to analyze the changes in viral pathogenicity. Real-time RT-PCR and IFN-γsecreting ELISPOT methods were used to analyze changes in characteristics of virology and immunology. Results During in vivo passage, CD4/CD8 ratio did not deeply decline. However,the peak and setpoint viral load in the line 3 show a continuous upward trend. The strong humoral and cellular immune responses were induced after SHIV-XJ02170 infection. Meanwhile, there was significant positive correlation between the viral load and binding antibody titer. Conclusion There were no pathogenic viral strains, and upward trend in virulence of SHIV-XJ02170 was found during in vivo passaging. SHIVXJ02170/Chinese origin Rhesus macaques model will play an important role in effect evaluation of candidate AIDS vaccines in China.