Antigen capture ELISA for specific detection of influenza A virus NS1 protein
10.3760/cma.j.issn.0254-5101.2010.06.018
- VernacularTitle:甲型流感病毒NS1抗原捕获ELISA的建立
- Author:
Xixia DING
- Publication Type:Journal Article
- Keywords:
Influenza A virus;
Nonstructural protein;
Monoclonal antibody;
ELISA
- From:
Chinese Journal of Microbiology and Immunology
2010;30(6):566-569
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a capture enzyme-linked immunosorbent assay (ELISA) for influenza A virus nonstructural protein NS1 using two anti-NS1 monoclonal antibodies(McAb) that map to distinct sites on the protein and to detect the NS1 in the culture supernatant and cell lysis supernatant of influenza virus. Methods Among the McAb against influenza A virus NS1, antibody pairs were analyzed to choose the optimal coating McAb and detecting McAb by determinating the detection sensitivity and specificity. Results The capture ELISA efficiently detected as little as 240 pg/ml of recombinant NS1 protein and exhibited no cross-reactivity for influenza B virus, parainfluenza virus NS1. Conclusion A sensitive and specific NS1-based capture ELISA for influenza A virus NS1 was successfully established, which could be an important tool for diagnosis of influenza A virus infection.
