CD4+ CD25high regulatory T cell frequency in cerebrospinal fluid of patients with neurosyphilis

VernacularTitle:神经梅毒患者脑脊液CD4+ CD25high调节性T细胞的研究
Author: Kang LI ; Haikong LU ; Xin GU ; Zhifang GUAN ; Hejun DAI ; Yihong QIAN ; Pingyu ZHOU
Publication Type:Journal Article
Keywords: Neurosyphilis; Cerebrospinal fluid; Regulatory T cells
From: Chinese Journal of Microbiology and Immunology 2010;30(6):528-531
CountryChina
Language:Chinese
Abstract: Objective To analyze the phenotypes of lymphocytes in cerebrospinal fluid derived from the patients with neurosyphilis. Methods Samples of cerebrospinal fluid from 12 patients with neurosyphilis and 20 patients with latent syphilis were collected and analyzed by flow cytometry for CD4 and CD25 expression. Results There was a significant increase in the number of white blood cells in the cerebrospinal fluid of patients with neurosyphilis. FACS analysis showed that most leukocytes were lymphocytes predominated with CD4 + T cells in neurosyphilis patients which were almost 4 times more than that in latent syphilis. However, there was a significant decrease in the proportion of CD4+ CD25high regulatory T cells (Tr) in neurosyphilis patients compared with that in latent syphilis patients. Conclusion A dramatic increase in CD4+ T cell frequency suggested its pathogenic role in neurosyphilis, whereas a decrease in CD25high Tr frequency indicated that CD4 + CD25high Tr cells might play an important role in immune homeostasis of central nervous system.