Establishment and identification of universal antibody against all subtypes of the influenza A viral hemagglutinins

VernacularTitle:抗甲型流感病毒血凝素通用抗体的制备及鉴定
Author: Changgui LI ; Juan LI ; Chun STELLA ; Ming SHAO ; Shuzhen LIU ; Xiaoyu CUI ; Xuguang LI ; Hanhua FANG ; Junzhi WANG
Publication Type:Journal Article
Keywords: Influenza A virus; Hemagglutinin; Universal antibody; Western blot
From: Chinese Journal of Microbiology and Immunology 2009;29(2):126-129
CountryChina
Language:Chinese
Abstract: Objective To establish the universal antibody against all subtypes of the influenza A viral hemagglutinins(HA). Methods All HA sequences from influenza viruses in common domains were analyzed to search for the most conservative re,on of the HA gene. The peptides conjugated to carrier proteins were used to immunize rabbits in order to obtain high titer antibody. Results The most conservative region among all influenza viruses HA genes were 14 amino acids located at the N-terminal of HA2. The antibody titer reached to 1 : 80 000 after 4 injections of the coupled peptides. The achieved antibody was demonstrated by Western blot to bind HA proteins of influenza virus subtypes H1-H13 specifically. Conclusion The universal antibody has been successfully established by immunizing the rabbits with most conservative peptides of HA protein coupled to carrier protein.