Prevalence of virulence genes in Streptococcus pneumoniae strains isolated from clinical patients

VernacularTitle:临床分离肺炎链球菌的毒力基因及血清型分析
Author: Yishan DONG ; Wenxiang HUANG ; Tao LUO ; Cheng ZHANG ; Lixian WU
Publication Type:Journal Article
Keywords: Virulence gene; Serotype; Streptococcus pneumoniae; Polymerase chain reaction
From: Chinese Journal of Microbiology and Immunology 2009;29(2):177-180
CountryChina
Language:Chinese
Abstract: Objective To investigate the prevalence of virulence genes(ply, pspA, nanA, lytA, psaA) among Streptococcus pneumoniae recently isolated from clinical patients. Methods The 133 strains were isolated from patients in three teaching hospitals in Chongqing from 2006 to 2008. Polymerase chain reaction was used to screen for virulence genes (ply, pspA, nanA, lytA, psaA). Results The positive rate of lytA, psaA, ply, nanA and pspA in 133 clinical isolates were 94.7%, 85.0%, 82.7%, 84. 2% and 60.2%, respectively. The positive rates of the lytA, psaA, ply, nanA and pspA genes in 87 common serotypes isolates was 100%, 87.4%, 86.2%, 89.7%, 67.8%, respectively. Conclusion The total positive rates of five virulence genes in the 133 clinical strains were high. The positive rates of five genes in the com-mon serotypes isolates were higher than those in the no-common serotypes. These genes are important virulence genes of Streptococcus pneumoniae. They could be candidates for protein vaccine of Streptococcus pneumoniae.