Detection of a new qnrA7 genotypes in Shewanella algae

VernacularTitle:海藻希瓦菌中发现一种新的qnrA7基因亚型
Author: Mingming ZHOU ; Hongxiang TU ; Tieli ZHOU ; Jingxian FEI ; Chao LI ; Yujie ZHAO ; Qiyu BAO
Publication Type:Journal Article
Keywords: Shewanella algae; Quinolone; Plasmid; qnrA7
From: Chinese Journal of Microbiology and Immunology 2010;30(7):593-596
CountryChina
Language:Chinese
Abstract: Objective To research the distribution and the characteristics of the plasmid mediated quinolone resistant genes in Shewanella algae. Methods The qnr, qepA, aac(6')-Ib-cr genes were amplified by PCR, then the positive PCR products were sequenced to determine the gene type. The transferability of plasmid mediated quinolone resistance was ensured by conjugation experiment. MICs were measured by E-test. qnrA gene was mapped to plasmids to locate it. Results The qnrA gene were detected in the Shewanella algae, this is a newfound subgroup qnrA7, the GenBank accession no. was GQ463707, qnrB, qnrS,qnrC, qnrD, qepA and aac(6')-Ib-cr genes were not detected. qnrA7 reside in a plasmid about 33 kb, conjugation experiment was unsuccessful. The strain was susceptible to quinolones. Conclusion It deserves paying close attention to the report of an original qnrA subgroup in an isolate of water-borne species of Shewanella algae.