Establishment of rat skin organ culture model and initiation of hair follicular morphogenesis with its correlative dynamic expression of Wnt10 b/β-catenin
10.3760/cma.j.issn.1671-0290.2010.02.013
- VernacularTitle:大鼠毛囊发育启动阶段模型构建及其Wnt10b/β连环蛋白信号表达的观察
- Author:
Yingchang JI
;
Yu LI
;
Feng LU
;
Zhiqi HU
;
Sen WANG
;
Changmin LIN
;
Jianhua GAO
- Publication Type:Journal Article
- Keywords:
Hair follicle;
Organ;
Culture;
Morphogenesis;
Wnt signal;
Wnt10b
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2010;16(2):116-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rat skin organ culture model to study the initiation of hair follicle morphogenesis, and the dynamic expression of Wnt10b/β-catenin in the developing hair follicle. Methods The dorsal skins of SD rat at embryos 14-17 (E14-E17) were cultured on a gelatin sponge-supported tissue culture system at the air/liquid interface of DMEM with 10 % fetal bovine serum (FBS) for 3-6 days, some of which removed from El5 were cultured in DMEM with FBS of different concentrations. HE staining and fluoroimmunoassay were adopted. Results The model we established allowed skin tissues isolated from E14-E15 to develop in a manner that was histologically and temporarily similar to the process in vivo. However, the developing hair follicle ceased to continue when their morphogenetic process reached the forth stage, and the concentration of FBS did not show any significant effect on the development of hair follicle. Expression of Wnt10b/β-catenin was induced in culture, as it also occurred in vivo,but grew weak till it disappeared in culture for 6 days, which was accompanied by development halt of hair follicle. Conclusions A rat skin organ culture model is established in which the morphogenesis of hair follicle takes place in a manner similar to in vivo, and Wnt10b/β-catenin probably has a close connection with the early morphogenesis of hair follicle.
