Expression and significance of regulatory genes of cell apoptosis in esophageal cancellation course

VernacularTitle:细胞凋亡相关基因在食管上皮癌变中的表达及其意义
Author: Lintao PENG ; Xin XU
Publication Type:Journal Article
Keywords: Esophageal neoplasms; Apoptosis; DNA; Flow cytometry
From: Cancer Research and Clinic 2010;22(7):473-475
CountryChina
Language:Chinese
Abstract: Objective To explore the expression of cell apoptosis regulatory elements in esophageal canceration course. Methods 60 specimens of esophageal carcinoma and corresponding adjacent no cancerous tissue were detected quantitatively by propidium iodide(PI) stain and indirect immunofluorescence of fluorescein isothiocyanate(FITC) using flow cytometry(FCM) equipment. Results Expression of bcl-2 and c-FLIP in esophageal carcinoma was significantly higher, and Fadd, Caspase-8 and Caspase-3 were significantly lower than that in adjacent tissues (P <0.01). There was positive correlation between Fadd and Caspase-8 gene (P <0.01 or P <0.05). The expression of Fadd was positively correlated to Caspase-8. Comparing with normal epithelium and dysplasia tissue, DNA content and heteroploid cells were increased significantly in esophageal carcinoma. Conclusion During the esophageal canceration course, expressions of relevant cell apoptosis regulatory elements were abnormal. DNA content and heteroploid cells were increased significantly in esophageal carcinoma.