Resveratrol protects against oxidative damage of fibroblasts irradiated with UVB
10.3760/cma.j.issn.0254-1424.2010.07.006
- VernacularTitle:白藜芦醇对中波紫外线照射致成纤维细胞氧化损伤的影响
- Author:
Yuehua YAN
;
Xin ZHOU
;
Tao SONG
;
Jianbo WU
;
Wanxiang SHENG
;
Xiaoming LIU
;
Chunrong YUAN
- Publication Type:Journal Article
- Keywords:
Ultraviolet B;
Resveratrol;
Dermal fibroblasts
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2010;32(7):503-505
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of resveratrol against oxidative damage to cultivated fibroblasts irradiated with UVB. Methods Fibroblasts from normal human skin cultured in vitro were divided into 5 groups (a normal control group, a group irradiated with UVB, a group treated with resveratrol before UVB irradiation, and a group treated after irradiation). A monolayer of fibroblasts was irradiated with UVB at 60 mJ/cm2. The vitality of the cells was measured using the methylthiazol tetrazolium (MTT) method. The activity of superoxide dis-mutase (SOD) and malondialdehyde (MDA) content were determined using enzyme biochemistry. Results Resveratrol over 100 μM inhibited the proliferation of fibroblasts. Resveratrol under 100 μM improved the proliferation of cells. The optimal concentration was 50 μM. UVB irradiation decreased the vitality of the cells and SOD activity, and it significantly enhanced MDA content. Conclusions Resveratrol treatment before or after UVB irradiation elevates the survival rate of fibroblasts, enhances the activity of SOD, and decreases MDA content. Resveratrol at low concentration could improve the proliferation of fibroblasts, and at high concentration could inhibit their proliferation. Res-veratol at 50 μM relieves the inhibited proliferation of fibroblasts damaged by UVB irradiation.
