An aptamer-based biosensor for colorimetric detection of Enteropathogenic Escherichia coli
10.3760/cma.j.issn.1009-9158.2010.07.003
- VernacularTitle:肠致病性大肠埃希菌比色检测的适配体生物传感器研究
- Author:
Wenhe WU
;
Yong CHEN
;
Luxi JIANG
;
Xiaoyang CUI
;
Xiaobo NIE
;
Jie ZHANG
;
Jianxin Lü
- Publication Type:Journal Article
- Keywords:
Enteropathogenic Escherichia coli;
Colorimetry;
SELEX aptamer technique;
Biosensing techniques
- From:
Chinese Journal of Laboratory Medicine
2010;33(7):587-593
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop and evaluate an aptamer based biosensor (aptasensor) for rapid colorimetric detection of enteropathogenic Escherichia coli (EPEC). Method The aptasensor was fabricated by modifying the truncated LPS-binding aptamer on the surface of nanoscale polydiacetylene vesicles using peptide bonding between the carboxyl group of the vesicle and the amine group of the aptamer. Molecular recognition between EPEC and aptamer at the interface of the vesicle led to blue-red transition of polydiacetylene which was readily visible to the naked eyes and could be quantified by colorimetric responses (CR). Transmission electron microscopy (TEM) was used to confirm the specific interactions between EPEC and polydiacetylene vesicles. Result Truncated aptamer showed the similar LPS-binding activity. The aptasensor could detect the target bacteria in a range of 105-108 colony-forming units (CFU)/ml within less than 30 minutes and its specificity was 100% for detection of EPEC O111. The sensor reproducibiliry obtained at 106 CFU/ml was 6. 08% R. S. D. The results of TEM confirmed that the specific interactions between EPEC and polydiacetylene vesicles. Conclusion A new aptasensor was developed successfully for rapid colorimetric detection of EPEC.
