Development of an HBV genotyping method by nucleic acid strip

VernacularTitle:核酸试纸条法检测HBV基因型方法的建立
Author: Kenü PAN ; Yongle ZHANG ; Hui ZHANG
Publication Type:Journal Article
Keywords: Nucleic acid; Hepatitis B virus; FQ-PCR; Genotype
From: Chinese Journal of Microbiology and Immunology 2010;30(8):768-770
CountryChina
Language:Chinese
Abstract: Objective To develop an rapid, visuable method to detect the genotypes of HBV.Methods According to the published full-length sequence with known genotypes in GenBank, the specific primer and biotin-lablled probe were designed. We established a nucleic acid strip method for genotyping hepatitis B virus(HBV) isolates among 150 HBV infected patients and 20 healthy controls, and compared the results with those obtained by real-time PCR. Results There was 34.00% genotype B , 61.33% genotype C, 4.00% genotype B/C in 150 samples, respectively, while the remaining 0.67% unknown. The results for this assay were high comparable to the method of real-time PCR. Conclusion With the similar sensitivity and specifity when compared with real-time PCR, this rapid method is suitable to the clinical setting.