Glycogen synthase kinase-3 in the lymphocytes of patients with mild cognitive impairment and Alzheimer's disease

VernacularTitle:轻度认知功能障碍及老年性痴呆患者外周淋巴细胞糖原合成酶激酶-3的变化
Author: Xingbin WANG ; Baiyuan ZHANG ; Qingde ZHANG ; Zhongsen QU
Publication Type:Journal Article
Keywords: Mild cognitive impairment; Alzheimer's disease; Lymphocytes; Glycogen synthase kinase-3
From: Chinese Journal of Physical Medicine and Rehabilitation 2010;32(7):523-526
CountryChina
Language:Chinese
Abstract: Objective To explore changes in glycogen synthase kinase-3 (GSK-3) levels in the lymphocytes of patients with mild cognition impairment (MCI) and Alzheimer's disease (AD). Methods GSK-3 activity was measured by 32P liquid scintillography in a control group, an MCI group and an AD group. The expressions of GSK-3βat Ser9 and total GSK-3β were determined by western blotting. Results GSK-3 levels were significantly higher in the AD and MCI groups than in the control group. The phosphorylation of GSK-3βat Ser9 was significantly lower in the AD and MCI groups compared with the control group, but there was no difference in the phosphorylation of total GSK-3β among the three groups. Conclusions Higher GSK-3 activity in the lymphocytes might have value in the diagnosis of MCI and early AD.