Effects of Different Scaffolds and Cultures on Growth of Preadipocytes
10.3760/cma.j.issn.1671-0290.2010.04.012
- VernacularTitle:不同支架及接种培养方法对前脂肪细胞生长情况的影响
- Author:
Jianfeng FEI
;
Hongquan SONG
;
Yang SUN
;
Rui ZHANG
;
Fengqin ZHANG
- Publication Type:Journal Article
- Keywords:
Preadipocyte;
Cell culture;
Tissue engineering;
Scaffolds
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2010;16(4):252-255
- CountryChina
- Language:Chinese
-
Abstract:
Objective To culture preadipocytes in vitro and to study the cell compatibility of PLGA scaffolds,collagen scarfolds and hyaluronic acid-based scaffolds and tO choose the optimal seeding method.Methods The preadipocytes from human abdominal adipose tissue were isolated and cultured in enzyme-digesting method.The generation of human preadipocytes was planted on PLGA scaffolds,collagen scaffolds and hyaluronic acid-based scaffolds.and the cell compatibility was observed by MTT method.The seeding efficiency of human preadipocytes on scaffolds.human preadipocytes were seeded to hyaluronic acid-based scaffolds by static culture and stirred culture.Results Compared compatibility of preadipocyte with three different scaffolds,there was great difference between hyaluronic acid-based scaffolds and PLGA scaffolds.Difference also existed between hyaluronic acid-based scaffolds and collagen scaffolds that were different from PLGA scaffolds.Among them,hyaluronic acid-based scaffolds was the best.Conclusion Hyaluronic acid iS a better scaffolds material for adipose tissue engineering compared with PLGA and collagen.The seeding efficiency of stirred culture is higher than static culture,which is an optimal method for cell seeding tO 3-D scaffolds.
