Effects of hirudin on cell circle of fibroblasts in hypertrophic scar
10.3760/cma.j.issn.1671-0290.2010.04.011
- VernacularTitle:水蛭素对人增生性瘢痕成纤维细胞细胞周期的影响
- Author:
Kaiheng ZHANG
;
Xin XING
- Publication Type:Journal Article
- Keywords:
Hypertrophic scar;
Fibroblasts;
Hirudin
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2010;16(4):248-251
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the biological effects of hirudin on the cell circle of fibroblasts in hypertrophic scar.Methods Fibroblasts were taken from normal skin and hypertrophic scar,and cultured in vitro with various concentrations of hirudin.Then,flow cytometry was used for observing cellcycles,and Western blot for some proteins (p27 and cyclin E) related with cell circle.Results With the increasing of the concentrations of hirudin,fibroblasts on G1 phase increased and on S phase decreased,and the expression of protein p27 increased,but that of protein cyclin E decreased.Conclusion Hirudin can influence the cell circle of fibroblasts derived from normal skin and hypertrophic scar by means of the expression of some proteins related with celI circle.Hirudin causes G1 phase arrest and inhibits the production of fibroblasts.Therefore,it seems that hypertrophic scar could be prevented and treated with hirudin.
