The application of monoclonal antibody 2A10 on protein chip for detecting of Flavivirus

VernacularTitle:单克隆抗体2A10在蛋白芯片技术检测黄病毒属病毒中的应用
Author: Tingting SUN ; Yuchang LI ; Hong LIU ; Xiaoping KANG ; Fang LIN ; Qingyu ZHU ; Yinhui YANG ; Cheng LU
Publication Type:Journal Article
Keywords: Protein chip; High-flux; Flavivirus; Monoclonal antibody
From: Chinese Journal of Microbiology and Immunology 2010;30(8):775-778
CountryChina
Language:Chinese
Abstract: Objective To evaluate the Flavivirus specific monoclonal antibody(McAb) 2A10 as detective antibody for simultaneously identify tick borne encephalitis virus( TBEV), Japanese encephalitis virus( JEV), dengue ( DEN )-2, DEN-4 and yellow fever virus ( YFV ) by antibody microarray technique.Methods The antibody microarray was developed by spotting TBEV, JEV, DEN-2, DEN-4 and YFV specific McAb on chip as capture antibodies. After incubating with cultured viral supernatants of the above viruses, CY3 labeled detective antibody 2A10 was added to the chips. After reaction, the antibody microarray was scanned and the results were analyzed. By comparing the signal intensities of different spots on chips,the detecting titre and sensitivity of 2A10 for Flavivirus were determined, and the value of 2A10 in detection of Flavivirus was evaluated. Results The hybridization results demonstrated that the titre of 2A10 for Flavi2A10 was specific for Flavivirus and could be used as universal detective antibody for Flavivirus on antibody microarray.