Different expression of microRNA-155 and cysteine-rich 61 in human placentas from severe preeclamptic and normal pregnancies
10.3760/cma.j.issn.1007-9408.2010.05.014
- VernacularTitle:重度子(癎)前期和正常妊娠胎盘中微小RNA-155及富含半胱氨酸蛋白61的差异表达
- Author:
Zhiqun WANG
;
Li SU
;
Zhenyu DIAO
;
Jianjun ZHOU
;
Yimin DAI
;
Yanqing ZHANG
;
Yali HU
- Publication Type:Journal Article
- Keywords:
Pregnancy;
Pre-eclampsia;
MicroRNAs;
Cysteine-rich protein 61;
Placenta
- From:
Chinese Journal of Perinatal Medicine
2010;13(5):403-407
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the different expression of microRNA-155 (miR-155) and cysteine-rich 61 (CYR61) in human placentas between severe preeclamptic and normal pregnancies.Methods Placentas were obtained from severe preeclamptic and healthy control pregnant women (n=18 for each group) at 36～40 gestational weeks. The expressions of miR-155 and CYR61 mRNA were assessed by real-time quantitative reverse transcription-polymerase chain reaction, and the levels of CYR61 protein were tested by Western blot. Results Compared with the control group, the miR-155 expression was increased in placentas from severe preeclampsia groups ( 165. 7 ± 16. 4 vs 527.9±49.1,t=7.00, P＜0.01), and the CYR61 mRNA expression (31.7±2.7 vs 16.4±1.2,t=5.10,P＜0. 01), as well as the CYR61 protein expression (36.4±1.5 vs 19.7±1.2,t=36.26, P＜0.01 ) were decreased. There was a significantly negative correlation between the expression of miR-155 and CYR61 mRNA within both groups (preeclamptic group: r=-0.52, P＜0.05;control:r=-0.57, P＜0.05). Conclusions Up-regulation of placental miR-155 in severe preeclampsia may be related to the decreased expression of CYR61. Both miR-155 and CYR61 may contribute to the disorders of placental angiogenesis in severe preeclampsia in human.
