The role of IL-27 in patients with primary biliary cirrhosis and its clinical significance
10.3760/cma.j.issn.0254-5101.2010.08.015
- VernacularTitle:IL-27介导的炎症反应在原发性胆汁性肝硬化中的作用
- Author:
Zhijun HAN
;
Yi SUN
;
Zihe YAN
;
Anmei DENG
;
Tingwang JIANG
;
Zhide HU
;
Yuanlan HUANG
;
Renqian ZHONG
- Publication Type:Journal Article
- Keywords:
Primary biliary cirrhosis;
Interleukin-27;
Autoimmune
- From:
Chinese Journal of Microbiology and Immunology
2010;30(8):755-759
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of IL-27 in PBC (primary biliary cirrhosis)patients and the possible involvement of IL-27 signal pathway in PBC. Methods The gene transcription and protein expression levels of IL-27 in patients with PBC, chronic hepatitis B(CHB) group and healthy controls(HC) were measured by real-time PCR, ELISA, flow cytometry and immunohistochemistry. AST,ALP, ALT, TBIL, GGT were determined and their correlation with IL-27 was also analyzed. Results IL-27 was significantly elevated in patients with PBC and IL-27 is present in the liver tissues of patients with PBC. Expression of IL-27 on CD4+T cells was increased in patients with PBC(72.40% ±6.22% ) and CHB(59.40% ± 7.03%) compared with HC(1.70%±0.55%,P＜0.01). Expression of IL-27 protein was increased in patients with PBC [( 126.25 ± 36.00 ) pg/ml] compared with CHB [( 51.81 ± 23.30 )pg/ml, P ＜ 0. 01] and HC[(34.19 ± 9.70) pg/ml, P ＜ 0.01], and it was positively correlated with GGT( r = 0.554, P＜0.01) and TBIL (r = 0.559,P＜0.01), but no correlation with ALT, AST, ALP.Conclusion These facts indicated the key role of IL-27 in the immune inflammatory reaction in patients with PBC.
