The influence of acupuncture on neural stem cell proliferation and differentiation in the hippocampus of the senescence-accelerated mouse prone-8
10.3760/cma.j.issn.0254-1424.2010.09.008
- VernacularTitle:针刺对快速老化小鼠海马神经干细胞增殖、分化的影响
- Author:
Xiaohong LI
;
Shengfeng LU
;
Xiulan QIAO
;
Haiyan YIN
;
Fang ZENG
;
Mei HUANG
;
Shuguang YU
;
Yong TANG
- Publication Type:Journal Article
- Keywords:
Acupuncture;
Senescence-accelerated mouse prone-8;
Hippocampus;
Proliferation;
Differentiation
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2010;32(9):662-665
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of acupuncture therapy on neural stem cell (NCS) proliferation and differentiation in the hippocampus of the senescence-accelerated prone-8 (SAP8) mouse. Methods Twenty-four SAP8 mice were randomly and equally divided into a model group and an acupuncture group. Twelve senescence-accelerated resistant ( SAR1 ) mice served as the control group. Acupuncture was administered at the Baihui (DU20) point to mice in the acupuncture group once daily for 21 consecutive days. Bromodeoxyuridine (BrdU) was used to detect the proliferation and differentiation of NCSs in the hippocampus through double-labeled immunofluoresSAR1 group, the positive cells in the SAP8 group were significantly fewer Compared to the SAP8 group, positive cells markers of neuron or glia cell positive cells appeared. Compared to the SAR1 group, the expression of BrdU/GFAP increased in the SAP8 group, and decreased after acupuncture but not significantly. BrdU/S-100 β cells decreased significantly in the SAP8 group, and increased after acupuncture, but again not significantly. Compared to the SAR1 group BrdU/GalC positive cells increased significantly in the SAP8 group and decreased significantly after acupuncture.Conclusions After acupuncture treatment for 21 days, the differentiation of hippocampal NSCs into oligodendrocytes was inhibited, but there was little effect on their differentiating into neurons and immature astrocytes.
