Analysis of the differential expression of Stathmin in HEK293 cells infected with human-tropic porcine endogenous retrovirus
10.3760/cma.j.issn.0254-5101.2010.09.005
- VernacularTitle:嗜人性猪内源性反转录病毒感染性克隆感染人胚肾细胞HEK293后Stathmin蛋白差异表达分析
- Author:
Qipo YAN
;
Yuyuan MA
;
Maomin Lü
;
Xiaoli YE
;
Lin ZHENG
;
Jianmin WU
;
Kegong TIAN
;
Jingang ZHANG
- Publication Type:Journal Article
- Keywords:
Porcine endogenous retrovirus;
HEK293 cells;
Stathmin;
Differential expression
- From:
Chinese Journal of Microbiology and Immunology
2010;30(9):804-808
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the differential expression of Stathmin in human cells infected with human-tropic porcine endogenous retrovirus(PERV)and to explore the potential molecular effect of human-tropic PERV on human cells.Methods HEK293 cells were infected with the human-tropic PERV infectious molecular clone.PCR,real-time RT-PCR and immunofluorescence analysis were applied to confirm that HEK293 cells were infected.Then real-time RT-PCR and Western blot were carried out to analyze the differential expression of Stathmin at the mRNA level and protein level,respectively.Results HEK293 cells were infected by human-tropic PERV.Real-time RT-PCR and Western blot analysis showed that Stathmin was up-regulated in HEK293 cells infected with PERV compared with the control cells.Conclusion Stathmin was up-regulated in HEK293 cells infected with human-tropic PERV.These studies will be helpful for revealing the interaction of PERV and human cells,and for understanding the molecular effect of humantropic PERV on human cells.In addition,it suggested that PERV infection may infect cell growth and physiological functions,even be pathogenic.These will help to clarify the biologic characteristics of PERV and evaluate the safety of PERV in pig to human xenotransplantation.
