B lymphocyte counts and Toil-like receptor 9 mRNA expression on cell from HIV-1 infected patients

VernacularTitle:HIV/AIDS患者外周血B细胞数量以及TLR9 mRNA表达水平的研究
Author: Hong SUN ; Wenqing GENG ; Hualu CUI ; Min ZHAO ; Xiaoxu HAN ; Zining ZHANG ; Jing LIU ; Di DAI ; Yanan WANG ; Yongjun JIANG ; Hong SHANG
Publication Type:Journal Article
Keywords: HIV; AIDS; B lymphocyte; Toll-like receptor
From: Chinese Journal of Microbiology and Immunology 2010;30(9):860-863
CountryChina
Language:Chinese
Abstract: Objective To study the B lymphocytes counts and the expression of TLR9 mRNA on B lymphocytes in peripheral blood from Chinese HIV-infected patients.Methods The cells from peripheral blood were stained with antibodies labeled with fluorescence and B lymphocytes were counted with flow cytometry.Using the method of magnetic activated cell sorting and real-time PCR,the expression of TLR9 mRNA was measured.Results The B lymphocytes counts in HIV/AIDS patients was significantly lower than that of healthy controls(P ＜0.01).The B lymphocytes counts in HIV/AIDS patients positively correlated with the CD4 +T cells counts(r =0.534,P = 0.006).The expression of TLR9 mRNA on B lymphocytes in HIV/AIDS patients was significantly lower than that of healthy controls(P =0.023),and positively correlated with the CD4 + T cells counts(r = 0.390,P = 0.040).Conclusion The B lymphocytes counts and the expression of TLR9 mRNA on B lymphocytes in Chinese HIV/AIDS patients were decreased due to HIV infection,which may correlate with disease progression.