Different cell death of THP-1 induced by virulent/attenuated purfied protein derivatives tuberculin and the different expression of TNF-α,IL-1β and IL-10 in Mycobacterium tuberculosis
10.3760/cma.j.issn.0254-5101.2010.09.012
- VernacularTitle:BCG-PPD、H37Rv-PPD诱导入巨噬细胞死亡及TNF-α、IL-1β和IL-10的表达差异
- Author:
Huilian SHI
;
Chanyi LU
;
Shenglei YU
;
Wenhong ZHANG
;
Xinhua WENG
;
Shu CHEN
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
THP-1;
Cell death
- From:
Chinese Journal of Microbiology and Immunology
2010;30(9):834-837
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the different response in macrophages treated with different agoβ and IL-10 in Mycobacterium tuberculosisnists(H37Rv-PPD and BCG-PPD)related with Mycobacterium tuberculosis and the relationship with TNF-αt,IL-1β and IL-10.Methods Using H37Rv-PPD and BCG-PPD to stimulate THP-1 cell for 3h,8h,15h,24h respectively.Cells were ananlyzed by Hochest staining under fluorescence microscopy to assay cell death(apoptosis and necrosis).At each stimulating time,TNF-α,IL-1β and IL-10 were examined by ELISA.Results Under fluorescence microscopy,it could easily see oval apoptotic bodies of THP-1 stimulated by BCG-PPD.However ,the nucleus were often isolated and necrosis-like when cells were stimulated by H37Rv-PPD.In a word ,BCG-PPD tend to induce THP-1 cells to apoptosis,but H37Rv-PPD inclined to induce cells to undergo necrosis.In supernatant of cells stimulated by BCG-PPD,the expression of TNF-αand IL-10 were lower than the cells stimulated by H37Rv-PPD,but the expression of IL-1β was higher than the latter.Conclusion It indicated that the necrosis of cells stimulated by H37Rv-PPD was asossiated with the excessive expression of TNF-α and IL-10,and the apoptosis of cells induced by BCG-PPD was IL-1β related.Perhaps the mechanism of differences in virulence exist in protein of strain,and associated with cytokines IL-1β,TNF-α and IL-10.
