Changes of plasma marinobufagenin level and its receptor Na+-K+-ATPase expression in kidney of chronic glomerulonephritis patients
10.3760/cma.j.issn.1001-7097.2010.09.001
- VernacularTitle:慢性肾炎患者血浆海蟾蜍毒素水平及其肾脏受体表达变化
- Author:
Jing CHEN
;
Yu WANG
;
Jiang TIAN
;
Zijian XIE
;
Xiaomei LI
- Publication Type:Journal Article
- Keywords:
Glomerulonephritis,chronic;
Marinobufagenin;
Na+-K+-ATPase
- From:
Chinese Journal of Nephrology
2010;26(9):653-656
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of plasma marinobufagenin (MBG) level and the expression of its receptor Na+-K+-ATPase (NKA) in renal biopsy specimens of chronic glomerulonephritis (CGN) patients. Methods Twenty-eight CGN patients and 14 healthy people were enrolled in the study. The plasma MBG concentration was measured by competitive inhibition ELISA system. Immunofluorescence and immunohistochemistry staining were applied to detect the expression of NKA in renal biopsy specimens of 28 CGN patients and analyzed by semi quantitively. Results Compared with healthy controls, CGN patients had significant lower plasma MBG concentration [(0.579±0.214) nmol/L vs (0.715±0.154) nmol/L, P＜0.05], without further significant difference between CGN patients with hypertension and with normal blood pressure [(0.595±0.231) nmol/L vs (0.557±0.197) nmol/L, P＞0.05]. Meanwhile, proximal tubular staining of NKA was decreased compared with normal controls. The NKA positive staining area of the CGN group was lower than that of normal controls [2.1% (0.5%-6.2%) vs 5.6% (3.5%-10.8%), P＜0.01] and correlated with 24-hour urinary sodium excretion (r=0.551, P＜0.01).Conclusion Decreased plasma MBG level and its receptor expression on proximal tubules may play a role in the regulation of sodium in CGN.
