The impact of Akt and nuclear factor κB pathway on chemoresistance of gastric cancer cell
10.3760/cma.j.issn.0254-1432.2010.08.012
- VernacularTitle:Akt和核因子κB信号通路在胃癌细胞化学治疗耐药中的作用
- Author:
Liangliang YU
;
Ning DAI
;
Honggang YU
;
Jieping YU
;
Jianmin SI
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Chemotherapy;
Drug resistance
- From:
Chinese Journal of Digestion
2010;30(8):550-555
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of Akt and nuclear factor (NF)-κB pathway in the development of chemoresistance in gastric cancer and the relation between Akt and NF-κB.Methods SGC-7901 cells were exposed to chemotherapeutic drugs (doxorubicin and etoposide ) or chemotherapeutic drugs combined with Wortmannin or MG-132.The cell growth was detected using MTT method.The apoptosis of SGC-7901 cells was measured by TUNEL and Annexin V/PI methods.The protein level of NF-κB was analyzed by immunocytochemical staining.Electrophoretic mobility shift assay (EMSA) was used to confirm the increased nuclear translocation of NF-κB/P65.chemotherapeutic drugs could obviously inhibit the growth of SGC-7901 cells in time-dose-dependent manner.Pretreatment of SGC-7901 cells with Wortmannin or MG-132 could promote this inhibitory κB in a dose-dependent manner.Wortmannin or MG-132 pretreatment could enhance the apoptosis of NF-κB was found in SGC-7901 cells stimulated with Wortmannin,but no activation of Akt was noted in those treated with MG-132.Conclusions The chemotherapeutic drugs can both induce apoptosis and activate Akt and NF-κB in SGC-7901 cells.The efficacy of chemotherapeutic drugs can be increased via inhibiting activation of Akt or NF-κB.
