The protective effects of histone deacetylases inhibitor TSA on the mice model of rheumatoid arthritis
10.3760/cma.j.issn.0254-5101.2010.09.001
- VernacularTitle:组蛋白去乙酰化酶抑制剂TSA对小鼠胶原诱导性关节炎的治疗作用
- Author:
Xin HUA
;
Yonghua BIAN
;
Xiaolei SUN
;
Yuhong JI
;
Jie ZHANG
;
Xiaoying WANG
;
Xiaorong ZHOU
- Publication Type:Journal Article
- Keywords:
Rheumatoid arthritis;
Trichostatin A(TSA);
IFN-γ;
IL-4
- From:
Chinese Journal of Microbiology and Immunology
2010;30(9):785-790
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of trichostatin A(TSA)on the mice model of collagen induced arthritis(CIA).Methods Mice model of rheumatoid arthritis(RA)was induced in DBA/1 mice with type Ⅱ collagen.Paws were scored for histological severity of arthritis.The severity of inflammation of mouse joint was evaluated by histological examination.Real-time PGR was used to determine the cytokine mRNA expression.Cytokine production was measured by ELISA from serum,spleen cell culture or dendritic cell and T cell co-culture supematant.T cell proliferation was examined by MTT method.Results TSA can significantly suppress the severity of the arthritis in CIA.IFN-γ was elevated in CIA mice,but was inhibited significantly by TSA introduced either at the same time with immunization or at the onset of manifestation of arthritis.Collagen specific T cell proliferation was significantly suppressed by introduction of TSA.Increased level of IL-4 by T cells was observed in TSA treated group compared to that of control group.Conclusion IL-4 level was increased and played a critical role in the protective effects of TSA in CIA.TSA suppresses the progress of CIA by regulates the balance of Th1/Th2 differentiation.
