Evaluation of cellular immune responses in mice elicited by Chinese AIDS candidate vaccines
10.3760/cma.j.issn.0254-5101.2010.09.013
- VernacularTitle:我国不同艾滋病候选疫苗小鼠细胞免疫应答评价
- Author:
Weijin HUANG
;
Chuntao ZHANG
;
Chenyan ZHAO
;
Jianhui NIE
;
Aijing SONG
;
Fengmin LU
;
Youchun WANG
- Publication Type:Journal Article
- Keywords:
AIDS;
Vaccine;
Cellular immune responses
- From:
Chinese Journal of Microbiology and Immunology
2010;30(9):838-842
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare cellular immune responses in mice elicited by Chinese different AIDS candidate vaccines.Methods According to their different immunization procedures,BALB/c mice were immunized with 6 AIDS candidate vaccines,separately.Spleen cells were isolated for the detection of cellular immune response to HIV-specific peptides using enzyme-linked immunosorbent spot(ELISPOT)assay and intracellular cytokine staining(ICS)method.Results AIDS vaccines were evaluated by using potential T-cell epitopes(PTE)Gag,Env and Pol peptides pool and ELISPOT.The positive conversion rates for cellular immune response of 1#-6# vaccines fluctuated from 70% to 100%.The vaccine-induced cellular immune responses to specific peptides pool are different not only in magnitude but also in breadth.The Th1type cytokines,IFN-γand IL-2,were detected with ELISPOT in 1# and 2# vaccines.The productions of IFN-γand IL-2 induced by both of the two vaccines showed a moderate correlation(r1 =0.62,P1 ＜0.01 ;r2=0.79,P2 ＜ 0.01).The positive conversion rate of IFN-γ secreting cells of 1 # vaccine was 66.7%(10/15)mice detected with both ELISPOT and ICS.And the results tested by ELISPOT and ICS showed moderate correlation(r = 0.55,P ＜ 0.05).Conclusion The magnitude and breadth of cellular immune responses induced by different AIDS candidate vaccines are different.Being induced by different AIDS candidate vaccines,the IFN-γand other Th1 type cytokines detected by ELISPOT or ICS could be used to evaluate the cellular immune responses in mice.
