Effects of chronic renal failure rabbit serum on proliferation and nuclear factor kappa B activation of rabbit arterial smooth muscle cells
10.3760/cma.j.issn.1001-7097.2010.09.011
- VernacularTitle:慢性肾衰竭兔血清对主动脉平滑肌细胞增殖及核因子κB活化的作用
- Author:
Yaoquan ZHANG
;
Fahuan YUAN
;
Bing FENG
;
Hong HU
- Publication Type:Journal Article
- Keywords:
Renal failure,chronic;
Serum;
Myocytes,smooth muscle;
Cell proliferation;
NF-kappa B
- From:
Chinese Journal of Nephrology
2010;26(9):696-701
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of chronic renal failure rabbit serum on proliferation and nuclear factor kappa B (NF-κB) activation of rabbit arterial smooth muscle cells (ASMCs) and to explore the possible mechanism. Methods Rabbit model of chronic renal failure was established by the ligation of renal arterial branches. ASMCs were incubated in the media with various concentrations of chronic renal failure serum cultured in vitro. Cell proliferation was assessed by MTT. Cell apoptosis was detected by Hoechst33342 staining. NF-κB p65 nuclear translocation was analyzed by immunofluorescence. Expression of proliferating cell nuclear antigen (PCNA) and NF-κB p65 proteins in response to chronic renal failure serum in ASMCs was determined by Western blotting. Results Lower concentrations of chronic renal failure serum (≤ 10%) could significantly promot the proliferation of ASMCs in a dose- and time-dependent manner. Higher concentrations of chronic renal failure serum (＞10%) could significantly inhibit the proliferation and induce apoptosis of ASMCs compared to the normal control (P＜0.05). Under the stimulation of lower concentrations of chronic renal failure serum, the expression of PCNA and NF-κB p65 increased significantly compared to the normal control (P＜0.01), while decreased markedly under the stimulation of higher concentrations of chronic renal failure serum compared to the normal control (P＜0.01). Under the stimulation of 10% chronic renal failure serum, nuclear translocation of NF-κB p65 in ASMCs was found. Conclusions Different concentrations of chronic renal failure rabbit serum can effectively induce ASMCs proliferation or apoptosis. The mechanism of promoting proliferation may be mediated by activating NF-κB, which will be useful for the treatment of accelerated atherosclerosis in chronic renal failure.
