Role of mitochondrial respiratory chain in hyperpermeability of human peritoneal mesothelial cells induced by high glucose peritoneal dialysate
10.3760/cma.j.issn.1001-7097.2010.10.013
- VernacularTitle:线粒体呼吸链在高糖腹膜透析液诱导人腹膜间皮细胞层高通透性中的作用
- Author:
Xuejing ZHU
;
Guanghui LING
;
Li XIAO
;
Youming PENG
;
Hong LIU
;
Yinghong LIU
;
Lin SUN
;
Fuyou LIU
- Publication Type:Journal Article
- Keywords:
Peritoneal dialysis;
Cell membrane permeability;
Reactive oxygen species;
Peritoneal mesothelial cell;
Mitochondrial respiratory chain
- From:
Chinese Journal of Nephrology
2010;26(10):785-790
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of mitochondrial respiratory chain in the hyperpermeability of human peritoneal mesothelial cells (HPMCs) induced by high glucose peritoneal glucose PDS was also added. Transmesothelial electrical resistance (TER) measurement was examined for detection of permeability damage in HPMCs. Immunostaining and Western blotting analysis were used to detect claudin-1 expression. Mitochondrial superoxide (MitoSOX) Red staining and respiratory chain complexes activities were determined for detection of mitochondrial reactive oxygen species (ROS) production and mitochondrial complexes activities. Results TER was decreased in a time- and concentration-dependent manner after culture with high glucose PDS for was also down-regulated significantly by high glucose PDS (P＜0.01). Complex Ⅲ activity was inhibited (10.8% of control, P＜0.01) accompanied with increased mitochondrial ROS generation.These changes were partially prevented by glutathione. Conclusion Mitochondrial respiratory complex Ⅲ pathway has crucial importance in maintaining TER of HPMCs, which may reveal a valuable target for novel therapies to fight hyperpermeability of peritoneum during the prolonged PD treatment.
