Study on the anti-tumor effects of the sonicate sensitized DC vaccine in vitro
10.3760/cma.j.issn.1006-9801.2010.10.005
- VernacularTitle:超声抗原树突状细胞瘤苗抗肿瘤效应的体外研究
- Author:
Yanyun XIE
;
Weiping FAN
;
Na LI
;
Xiaojuan YUAN
;
Xia PENG
- Publication Type:Journal Article
- Keywords:
Neoplasms;
Cancer vaccines;
Dendritic cell;
T-lymphocyte,cytotoxic;
Immunologic;
SW480
- From:
Cancer Research and Clinic
2010;22(10):663-665
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the cytotoxicity of the cytotoxic T lymphocyte (CTL) induced by SW480 sonicate sensitized dendritic cells (DC) on the colon cancer cell line SW480. Methods PBMC were separated from the HLA-A*0201 donor and DC were cultured with rhGM-CSF, rhIL-4 and rhTNF-α. The same donor's primary CTL were stimulated by DC loaded with SW480 sonicate. The cytotoxicity of CTL on SW480 (HLA-A*0201 positive) and K562 (HLA-A*0201 negative) was determined by the MTT method. Results The cytotoxicity of the CTL on SW480 was stronger than that on K562 (P ＜0.05). Conclusion The DC vaccine can stimulate specific CTL which can trigger cytotoxic activity on the target cells and this cytotoxicity is related to MHC restriction.
