Detecting serum anti-aquaporin-4 antibody in neuromyelitis optica patients by mimic antigen based ELISA
10.3760/cma.j.issn.0254-5101.2010.10.021
- VernacularTitle:模拟抗原ELISA检测视神经脊髓炎患者血清水通道蛋白-4抗体
- Author:
Mangsuo ZHAO
;
Tongchao GENG
- Publication Type:Journal Article
- Keywords:
Neuromyelitis optica;
Devic's disease;
NMO-IgG;
Aquaporin-4
- From:
Chinese Journal of Microbiology and Immunology
2010;30(10):965-967
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore mimic antigen based ELISA for detecting serum anti-acquaporin4(AQP4) antibody in neuromyelitis optica(NMO) patients. Methods Three polypeptides: AQP456-69,AQP4135-155, AQP4209-230 were designed to simulate antigen epitopes of AQP4 through biological information and structure biology analysis, the peptides was used as antigen in ELISA to detect serum anti-AQP4 antibody in 9 NMO patients and 7 other miscellaneous neurological disorders which hayed been detected by immumofluorescence method. Results The mean value of A of anti-AQP4 antibody postive patients which have been determined by immumofluorescence method were higher than the controls in ELISA with AQP4135-155,AQP4209-230 as antigen (P<0.05). When the patients serum were diluted at 4 and 8 times, the A values were higher than controls significantly ( P<0.05 ). Conclusion Outmembrane polypeptides AQP4135-155,AQP4209-230 may be the main antigen epitope or main part of antigen epitope, they could be used to detect serum anti-AQP4 antibody in NMO patients.
