Study on silencing of endogenetic mdr-1 gene expression by RNA interference in ovarian cancer resistant strain SKOV3/TAXOL
10.3760/cma.j.issn.1006-9801.2010.10.013
- VernacularTitle:通过小干扰RNA沉默mdr-1基因逆转卵巢癌耐药细胞株SKOV3/TAXOL多药耐药的研究
- Author:
Jiliang FENG
;
Huanling YAO
;
Hongmei WANG
;
Xiuping DU
- Publication Type:Journal Article
- Keywords:
RNA interference;
Ovarian neoplasms;
Multidrug resistance protein-1;
P-glycoprotein;
Genes;
Cell proliferation;
Apoptosis
- From:
Cancer Research and Clinic
2010;22(10):686-688
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the small hairpinRNA recombinant plasmids targeting mdr-1 gene which expresses highly in ovarian cancer resistance strain SKOV3/TAXOL to silence endogenetic mdr-1 gene expression and investigate the role of mdr-1 gene in the development of resistant ovarian cancer. Methods The pPGPU6/GFP/Neo-mdr-1 were constructed by gene clone technology. The influence on proliferation and apoptosis were investigated by CCK-8 in SKOV3/TAXOL after transfected. pPGPU6/GFP/Neo-mdr-1. Results The expression against mdr-1 proteins were inhibited by pPGPU6/GFP/Neo-mdr-1. The cell proliferation were inhibited after transfected pPGPU6/GFP/Neo-mdr-1 by CCK-8. The apoptosis were observed in DAB experiments and the apoptosis rate incised. Conclusion mdr-1 plays an important role in proliferation of resistant ovarian cancer and the short hairpinRNA of mdr-1 can efficiently suppress mdr-1expression and enhance the apoptosis in SKOV3/TAXOL, which provides a novel method for chemotherapy resistant tumors.
