Sequence analysis of plasmid in Klebsiella pneumoniae KF3
10.3760/cma.j.issn.0254-5101.2010.10.003
- VernacularTitle:一株多重耐药肺炎克雷伯菌KF3的质粒研究
- Author:
Hongyun LU
;
Hongqin ZHANG
;
Xiaoding YAO
;
Junrong WANG
;
Yali XI
;
Mingming ZHOU
;
Tieli ZHOU
;
Qiyu BAO
;
Jinsong LI
- Publication Type:Journal Article
- Keywords:
Plasmids;
Drug resistant genes;
Conjugative transfer genes;
Klebsiella pneumoniae
- From:
Chinese Journal of Microbiology and Immunology
2010;30(10):892-896
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the structures of the plasmids of Klebsiella pneumoniae KF3 at the genome metagenome level througth with whole plasmid DNA sequencing, to analyze the functional genes carried by plasmid and to identify the correlation of resistance and pathogenicity between the plasmids and the host strains. Methods The alkaline lysis method was used to extract plasmids. We constructed the small insert pUC18 library and the large insert Forsmid library, sequenced and used the Phred / Phrap / Consed package to assemble these sequences and gained a complete sequence. The open reading frame(ORFs) were predicted by the Glimmer software and annotated, analyzed the functions of these genes. Results We successfully constructed the pUC18 library and the Fosmid libraries for the plasmid DNA and obtained three circular double-stranded DNA plasmids: pKF3-70 (69 477 bp), pKF3-90 (91 327 bp) and pKF3-147 ( 147 416 bp). There were drug resistant genes, conjugative transfer genes and mobile DNA elements identified on three plasmids. Conclusion The three plasmids of KF3 could be transferred among different strains. It would lead to the dissemination of the resistant genes.
