Subtype and sequence analysis of the gag and env genes for HIV-1 strains isolated in Hebei Province

VernacularTitle:河北省HIV-1流行株基因序列测定及亚型分析
Author: Cuiying ZHAO ; Qiaomin LI ; Hongru ZHAO ; Xinli LU ; Baojun LI ; Yuqi ZHANG ; Xiangfen MIAO ; Hui XING ; Xiang HE
Publication Type:Journal Article
Keywords: HIV-1; Genetic subtype; Sequence analysis
From: Chinese Journal of Microbiology and Immunology 2010;30(10):914-918
CountryChina
Language:Chinese
Abstract: Objective To study the epidemic situation and characteristic of subtypes of HIV-1 strains prevalent in Hebei Province. Methods Viral RNA was extracted from plasma samples, HIV-1 genes (env and gag ) were amplified by RT and nested-PCR using specific primer pairs and sequenced directly.The acquired sequences were compared with international subtypes references and their phylogenetic-trees were analyzed to determine the subtype. Results Among 154 HIV-1 antibody positive cases , 148 HIV-1 gene fragments were amplified and analyzed. There were 6 kinds of HIV-1 subtypes and recombinants, moreover unidentified 2 cases in 148 samples, of which 61 (41.2%) cases of B', 59(39.9% ) cases of CRF01_AE, 16( 10.8% ) cases of CRF07_BC, 6(4.1% ) cases of CRF08_BC, and 2( 1.4% ) cases of C and B01 each. HIV-1 B01 was detected firstly in Hebei Province. Conclusion Six HIV-1 subtypes were identified in Hebei Province. B' and CRF01_AE are the primary subtypes and recombinants of HIV-1 existed in Hebei Province. The surveillance of HIV-1 gene variation should be paid more attention to.