Effect of 1,25(OH)_2D_3 on the expression of vitamin D receptor, tumor necrosis factor a and transforming growth factor β1 in rat peritoneal mesothelial cells stimulated by lipopolysaccharide
10.3760/cma.j.issn.1001-7097.2009.11.010
- VernacularTitle:1,25(OH)_2D_3对脂多糖作用下大鼠腹膜间皮细胞维生素D受体及肿瘤坏死因子α、转化生长因子β1表达的影响
- Author:
Lina YANG
;
Jianfei MA
;
Shuo CHEN
;
Liyan LI
;
Lining WANG
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Calcitriol;
Receptors;
calcitriol;
Peritoneal dialysis;
Peritonitis;
Tumor necrosis factor-alpha;
Transforming growth factor betal
- From:
Chinese Journal of Nephrology
2009;25(11):856-861
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of 1,25(OH)_2D_3 on expression of vitamin D receptor (VDR), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1) in rat peritoneal mesothelial cells (RPMCs) stimulated by lipopolysaccharide (LPS), so as to provide some evidence for clinical use of 1,25 (OH)_2D_3 on peritoneal dialysis-associated peritoneal inflammation. Methods RPMCs were isolated, cultured and passaged by enzymaticdisaggregation, then identified by phase contrast inverted microscope, transmission electron microscope with immunocytochemistry method. RPMCs were incubated with LPS (1,10,100 mg/L) and LPS (10 mg/L) for 2,6,12 hours, or stimulated by 1,25(OH)_2D_3 (10~(-8) mol/L, 10~(-7) mol/L, 10~(-6) mol/L) after incubated with LPS (10 mg/L) for 2 hours. RPMCs in the control group were justincubated with medium. Expression of VDR mRNA and protein was detected by RT-PCR and Western blot. In addition, ELISA was performed to investigate the changes of TNF-α and TGF-β1 in culture medium. Results Compared with control group, the expression of VDR mRNA and protein was decreased in LPS group (P<0.05). LPS could significantly induce the expression of TNF-a and TGF-β1 in RPMC (P <0.01), which could be partially reversed by different concentrations of 1,25 (OH)_2D_3 (P<0.01). Conclusions 1,25 (OH)_2D_3 can reverse the decrease of VDR mRNA and protein induced by LPS as well as the induction of TNF-a and TGF-β1 up-regulated by LPS in RPMC in a dose- and time-dependent manner. Our research provides experimental evidence of VDR ameliorating peritoneal dialysis-associated peritoneal inflammation and peritoneal fibrosis.
