Study of primary cells of breast cancer with special immune response induced by CIK co-cultured with self-dendritic cells
10.3760/cma.j.issn.1006-9801.2010.12.007
- VernacularTitle:自体来源树突状细胞和细胞因子诱导的杀伤细胞混合培养对原代乳腺癌细胞的杀伤作用
- Author:
Hongli LIANG
;
Hongnan JIANG
;
Xiaojun LI
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Dendritic cells;
Cytokines;
Killer cells
- From:
Cancer Research and Clinic
2010;22(12):814-816
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effect to kill primary breast cancer cells by dendritic cell (DC)with self-freeze thawing antigens of primary breast cancer cells co-cultured with cytokine induced killer cell (CIK). Methods Self-neoplasm antigen by primary cells of breast cancer in log phase growth was prepared and DC and CIK cells was cultured from peripheral blood. The CIK of co-cultured DC loaded self-neoplasm antigen was compared to PBMC, CIK cells with self-neoplasm antigen and the single CIK cells. Results Killing efficiency of PBMC, CIK, Ag-CIK and Ag-DC-CIK were (34.35±3.28) %, (45.91±2.78) %, (50.88±3.22) %, (62.10±5.94) %. There were significant difference between Ag-DC-CIK group and CIK-Ag and CIK group to primary cells of breast cancer (P <0.01). Conclusion The CIK induced from cocultured DC loaded with self-neoplasm antigen show a special killing activity to self-primary tumor cells.
