Study on defective interfering particles of Paramyxovirus, Tianjin strain enhanced the immune response of the mice
10.3760/cma.j.issn.0254-5101.2010.11.020
- VernacularTitle:副黏病毒Tianjin株缺损性干扰颗粒增强小鼠免疫应答的初步研究
- Author:
Hongjing ZHOU
;
Jing ZHANG
;
Zhiyun CHEN
;
Fang WANG
;
Xiaomian LI
- Publication Type:Journal Article
- Keywords:
Paramyxovirus,Tianjin strain;
Defective interfering particles;
Poliovirus;
Immune response
- From:
Chinese Journal of Microbiology and Immunology
2010;30(11):1063-1067
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the defective interfering(DI) particles of Paramyxovirus, Tianjin strain could be used as a candidate adjuvant. Methods DI particles were separated and purified. After being identified, it was equally mixed with the inactivated poliovirus (PV) vaccine. Kunming mice were administered subcutaneously with the mixture, besides we set groups of inactivated PV vaccine containing Al(OH) 3 as an adjuvant, inactivated PV vaccine of which the dose was doubled without any adjuvant, and negative control. Sera were collected in the 14th day after immunization, and the specific antibodies of PV were detected. T/B lymphocyte stimulation indexes(SI) were counted through the lymphocyte proliferation tests. The quantities of IFN-γ/IL-4 produced by the splenocytes which were stimulated again by PV antigen were detected. Results The SI and the quantity of IFN-γof the mice in the group of inactivated PV vaccine combining with DI particles of Paramyxovirus, Tianjin strain were more than other groups. Conclusion DI particles of Paramyxovirus, Tianjin strain could enhance the immune response of inactivated poliovirus, especially the cellullar immunologic response of Th1 type.
