The effects of low frequency pulsed magnetic fields with different waveforms on cardiac microvascular endothelial cells
10.3760/cma.j.issn.0254-1424.2010.11.002
- VernacularTitle:不同波形低频脉冲磁场对大鼠心肌微血管内皮细胞的影响
- Author:
Nan LIU
;
Fei LI
;
Haichang WANG
;
Chen WANG
;
Yanhui SU
;
Xiaobo XIE
- Publication Type:Journal Article
- Keywords:
Low frequency pulsed magnetic fields;
Cardiac microvascular endothelial cells;
Proliferation;
Cell cycle;
Apoptosis;
Migration;
Cytoskeleton;
Nitric oxide
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2010;32(11):805-810
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of low frequency pulsed magnetic fields (LF-PMFs) on proliferation, the cell cycle, apoptosis, migration, cytoskeleton formation and nitric oxide (NO) secretion in cardiac microvascular endothelial cells (CMECs). Methods LF-PMFs with rectangular and triangular waveforms at 15 Hz were tested. The CMECs were randomly divided into a control group, 0.6 mT group, 1.2 mT group, 1.8 mT group and 2.4 mT group. Except for the control group, the CMECs were exposed to LF-PMFs 4 h/day for 5 days. Results After 5 days of intervention with rectangular wave LF-PMFs the proliferation of CMECs accelerated and NO secretion was enhanced significantly. The percentages of cells at ( S+G2 ) phase increased significantly, whereas apoptosis rates were significantly lower than in the control group. The migration of CMECs was facilitated, stress fibers increased and cytoskeleton components were reorganized. After 5 days of intervention with triangular wave LF-PMFs proliferation of CMECs accelerated and NO secretion was significantly enhanced. The percentages of cells at(S+G2)phase and migration increased, while apoptosis was inhibited. Cytoskeleton components were reorganized after exposure to 0.6 mT and 2.4 mT triangular waveform LF-PMFs. No significant change was detected with 1.2 mT and 1.8 mT triangular waveform LF-PMFs with regard to these variables. Conclusions The effects of LF-PMFs on proliferation, the cell cycle, apoptosis, migration, cytoskeleton formation and NO secretion function of CMECs were infiuenced by the waveform in addition to it's intensity and frequency.
