Effect of ulinastatin on expression of interleukin 15, connective tissue growth factor and malondialdehyde in rat peritoneal mesothelial cells induced by high glucose
10.3760/cma.j.issn.1001-7097.2010.12.006
- VernacularTitle:乌司他丁对高糖作用下大鼠腹膜间皮细胞白细胞介素15、结缔组织生长因子及丙二醛表达的影响
- Author:
Xia PENG
;
Jianfei MA
;
Chengcheng LI
;
Shuo CHEN
;
Pengpeng WANG
- Publication Type:Journal Article
- Keywords:
Peritoneal dialysis;
Interleukin- 15;
Malondialdehyde;
High glucose;
Peritoneal mesothelial cell;
Connective tissue growth factor;
Ulinastatin
- From:
Chinese Journal of Nephrology
2010;26(12):904-908
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of ulinastatin on the expression of interleukin 15 (IL-15), connective tissue growth factor (CTGF) and malondialdehyde (MDA) in rat peritoneal mesothelial cells (RPMCs) induced by high glucose. Methods RPMCs were isolated, cultured and passaged by trypsin, then identified. The third generation of cultured RPMCs were used in the experiment. RPMCs were divided into normal control group, high glucose (1.5%, 2.5%, 4.25%) for 6 hours and 12 hours, high glucose (2.5%) for 3, 6, 12, 24 hours or ulinastatin (160, 320, 640U/ml) for 12 hours. IL-15 mRNA was detected by real-time PCR. IL-15 and CTGF protein in supernatants was detected by ELISA. MDA protein was detected by TBAS. Results Compared with the control group, the expression of IL-15, CTGF and MDA was significantly increased in the groups stimulated by high glucose (P＜0.05) in dose- and time-dependent manner. Ulinastatin could significantly decrease the expression of IL-15, CTGF and MDA induced by high glucose in dosedependent manner both in protein and gene levels (P＜0.05). Conclusions High glucose can up-regulate the expression of IL-15, CTGF and MDA in RPMCs. Ulinastatin can reverse these changes.
