Effects of Livin antisense ologonucleotide on the proliferation and apoptosis of K562 cells

VernacularTitle:Livin反义寡核苷酸对K562细胞增殖和凋亡的影响
Author: Xiuhong JIA ; Shaohua XIE ; Jianchang LI ; Zhaodong HAN ; Yuanyuan LIU
Publication Type:Journal Article
Keywords: Oligonucleotides,antisense; Livin; K562; Cell proliferation; Apoptosis
From: Cancer Research and Clinic 2011;23(1):34-37
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of Livin antisense oligonucleotide (ASODN) on the proliferation and apoptosis of human leukemia (K562) cells. Methods Specific phosphorothioate ASODN and missense oligonucleotide (MSODN) target Livin mRNA were synthesized and transfected into K562 cells following cationic liposome. The proliferation inhibition of K562 cells was assessed by MTT. The apoptosis rate of each group was detected by Annexin V-FITC. The expression of Livin mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). Results ASODN at a final concentration of 600 nmol/Lcould inhibit the K562 cells proliferation (IR) was (52.99t2.67) % and the expressions of Livin mRNA (ODR)was (59.75±3.24) %, the apoptosis rate was apparently increased [(36.89±1.08) %] (P ＜0.01); but the difference between Lip-MSODN group, Lip control group and cell control group was not statistically significant (P ＞0.05).Conclusion Livin ASODN may decrease Livin gene expression, suppress K562 cells proliferation effectively, and induce significant apoptosis of K562 cells.