Study on species identification of brucella with small fragments of PCR
10.3760/cma.j.issn.1009-9158.2010.12.019
- VernacularTitle:小片段PCR产物对布鲁菌种属鉴定的研究
- Author:
Liyuan SUN
;
Bin LIU
;
Xuefeng Lü
;
Fan LI
- Publication Type:Journal Article
- Keywords:
Brucella;
Polymerase chain reaction
- From:
Chinese Journal of Laboratory Medicine
2010;33(12):1185-1188
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the species of brucella rapidly with small fragments of PCR products. Methods The primers were designed according to Brucella spp. repeated insertion sequence IS711 in addition to the specific gene sequences of Brucella melitensis, Brucella abortus and Brucella suis. The small fragments of 3 standard strains and 13 clinical isolates of Brucella were amplified by PCR. Then PCR products were T-A cloned and sequenced. The DNA of standard strains were diluted for sensitivity and stability testing. Results Four specific PCR products were obtained by PCR (63, 67, 81 and 83 bp). The results of T-A cloning and sequencing were in accord with the target gene fragment test. The detection limit of DNA was 1 μg/L. The expected results were achieved with different primers. Conclusion Species identification of Brucella with small fragments of PCR production is a method to identify Brucella strains with great specificity,sensitivity and stability.
