Combining CpG and Al(OH)3 enhances the immunogenicity of hepatitis C virus recombinant ptotein combined vaccine
10.3760/cma.j.issn.0254-5101.2010.12.010
- VernacularTitle:CpG和Al(OH)3佐剂联合使用增强丙型肝炎病毒重组蛋白疫苗的细胞免疫原性
- Author:
Guangxue LI
;
Bingshui XIU
;
Shigan LING
;
Heqiu ZHANG
;
Tao YAN
;
Lin WEI
;
Feng HE
;
Xiaoyan FENG
;
Xiaoguo SONG
;
Guohua WANG
;
Kun CHEN
;
Jing HE
;
Ruihong ZENG
- Publication Type:Journal Article
- Keywords:
Hepatitis C virus;
Recombinant protein combined vaccine(TFE);
Adjuvant;
CpG;
Al(OH)3
- From:
Chinese Journal of Microbiology and Immunology
2010;30(12):1110-1113
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research CpG and Al(OH)3 adjuvants enhancing immunogenicity of hepatitis C virus(HCV) recombinant ptotein combined vaccine(TIE).Methods BALB/c mice were immunized with candidate vaccine TFE using CpG,Al(OH)3,Al(OH) 3 + CpG,or freund's adjuvant(FA) as the adjuvant.Five mice were sacrificed after 10 d of the last immunization.Specific antibodies in sera were tested by enzyme-linked immunosorbent assay(ELISA).Splenic cells were isolated and levels of IFN-γ,IL-4 and cytotoxic T lymphocyte(CTL) cytotoxicity assay were messuredin vitro.The remaining mice were subcutaneouly injected with 1 × 106 SP2/0-NS3 cells on the back to investigate the protective effects.The differences of means between groups were compared by LSD-t test.Results The specific CTL activity of TFE + A1(OH) 3 + CpG group was higher than TFE + FA group and TFE + CpG group(P ＜ 0.05).The level of IFN-γsecreting cells in TFE + Al(OH)3 + CpG group was higher than that in TFE + M(OH)3 group or TFE + CpG group(P ＜ 0.05).Conclusion Combining Al(OH) 3 and CpG could enhance specific cellular immunogenicity of candidate HCV vaccine TFE.TFE + M(OH) 3 + CpG could effetively prevent the attack of tumor cell SP2/0-NS3 expressing nonstructural protein NS3 of HCV.
