Effects of erythropoietin on peripheral blood endothelial progenitor cells from rats with chronic kidney disease
10.3760/cma.j.issn.1001-7097.2010.11.008
- VernacularTitle:红细胞生成素对慢性肾衰竭大鼠外周血内皮祖细胞的影响
- Author:
Jianxin WAN
;
Xia YANG
;
Jiong CUI
;
Zhenhuan ZOU
- Publication Type:Journal Article
- Keywords:
Kidney failure,chronic;
Erythropoietin;
Erythroid progenitor cells;
Drug effects;
Hemangioblast
- From:
Chinese Journal of Nephrology
2010;26(11):840-844
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of erythropoietin (EPO) on the number and function of peripheral blood endothelial progenitor cells (EPCs) from rats with chronic renal failure (CRF). Methods The model of chronic renal failure was established by a two-stage 5/6nephrectomy procedure in rats. Experimental rats were randomly divided into four groups (n =7,respectively): sham operation group, CRF group, CRF rats treated with 30 U/kg EPO (low-dosage group) and with 50 U/kg EPO (high-dosage group). CRF rats were given EPO by hypodermic injection for 6 weeks, then EPCs were isolated by density gradient centrifugation from peripheral blood mononuclear cells. The ability of cell proliferation, adhesion and vasculogenesis in vitro was further observed. Results Compared to sham operation group, the ability of cell proliferation,adhesion and vasculogenesis in vitro in CRF rats was remarkably decreased (P＜0.05, respectively).Such ability was promoted significantly in dose-dependent manner by EPO treatment (P＜0.05,respectively). Conclusion EPO can improve the number and ability of endothelial progenitor cells from rats with chronic renal failure.
