Initial observation of Madin-Darby canine kidney cells being implanted on the polysilicon nanopore membrane by micro-electro-mechanical system
10.3760/cma.j.issn.1001-7097.2010.11.010
- VernacularTitle:狗肾脏细胞在微电子机械系统人工膜上生长的初步观察
- Author:
Shaojun LIU
;
Ling LIU
;
Mingxin LI
;
Yan WANG
;
Guifu DING
;
Jun XUE
;
Yong GU
;
Chuanming HAO
;
Shantan LIN
- Publication Type:Journal Article
- Keywords:
Nanomembrane;
Glomerular filtration membrane;
Tissue engineering
- From:
Chinese Journal of Nephrology
2010;26(11):851-856
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the growing shape and function of Madin-Darby canine kidney (MDCK) cells implanted on the polysilicon nanopore membrane by micro-electro-mechanical system (MEMS). Methods The polysilicon nanomembrane was made by silicon film processed via whirling photoresist, wet etching, electroplating and so on, and then it was coated by extracellular matrix and implanted with MDCK cells. The cell growth shape and function was observed or examined by scanning electron microscope or MTT test and Trypan Blue staining.Results The nanomembrane with regular slit pores was successfully fabricated. Extracellular matrix-coated nanomembrane, especially for collagen Ⅳ coating, was more suitable for MDCK cells to adhere and proliferate without membrane injury. The polysilicon nanomembrane coated with extracellular matrix did not induce the cell death and also not stimulate cells releasing the cytokines such as interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α). Under scanning electron microscope, MDCK cells formed a flat single-cell fusion with tight junction on the surface of polysilicon nanomembrane and there was a large number of microvillus on the top of cells.Conclusion The collagen-coated polysilicon nanomembrane made by MEMS techniques, with no cytotoxicity and good biocompatibility, is valuable to frame the artificial glomerular filtration membrane
