Evaluation of the performance of a DNA microarray method for detecting HBV mutations conferring antiviral resistance to lamivudine and adefovir
10.3760/cma.j.issn.1009-9158.2010.11.011
- VernacularTitle:DNA微阵列芯片法检测慢性乙型肝炎拉米夫定和阿德福韦酯耐药突变
- Author:
Ruifeng YANG
;
Shaocai DU
;
Xu CONG
;
Hui MA
;
Lai WEI
- Publication Type:Journal Article
- Keywords:
Hepatitis B,chronic;
Hepatitis B virus;
Oligonucleotide array sequence analysis;
Drug resistance,viral;
Sequence analysis,DNA
- From:
Chinese Journal of Laboratory Medicine
2010;33(11):1049-1053
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the performance of a DNA microarray method for detecting HBV antiviral drug-resistant mutations. Methods Two hundred and twenty four serum samples from patients with CHB were tested in parallel by DNA microarray and direct sequencing for the mutations within the HBV reverse transcriptase (rt) region, which included rtL180, rtA181, rtM204 and rtN236. Samples with discrepant results were retested by clonal sequencing. Results Complete concordance between DNA microarray and direct sequencing results was observed in 214 out of 224 samples (95. 5% ). The presence of mixed viral populations in the other 10 samples detected by DNA microarray but not by direct sequencing was confirmed by clonal analysis. The DNA microarray could detect minor viral populations which constituted 5.0%-15. 0% of the total viral load. Conclusion DNA microarray is highly consistent with direct sequencing in detecting HBV mutations conferring drug resistance and more sensitive in detecting mixed mutant and wild-type sequences than direct sequencing, which makes it a useful tool for early detection of drug resistance early.
