Subtype and sequence analysis of gag genes in HIV-1 circulating in sexual infectors in Beijing

VernacularTitle:北京市性传播HIV-1感染者流行毒株gag基因序列测定和亚型分析
Author: Jingrong YE ; Lei GUO ; Lishi BAI ; Ruolei XIN ; Hongyan LU ; Shuangqing YU ; Yi ZENG
Publication Type:Journal Article
Keywords: Human immunodeficiency virus; Subtype; Nested-PCR
From: Chinese Journal of Microbiology and Immunology 2011;31(2):136-139
CountryChina
Language:Chinese
Abstract: Objective To investigate the subtype distribution and sequence characteristics of HIV-1 strains prevalent among sexual infectors in Beijing. Methods We collected the blood samples from 100HIV sexual infectors in Beijing during 2008 and separated plasma specimens. RNA was extracted from the plasma and the gag gene was amplified by RT-PCR and nest-PCR. The PCR products were sequenced directly and phylogenetic analyses of gag gene was performed using the MEGA4 software. Results Among 100 HIV-1 plasma samples,84 gag gene fragments were amplified and analyzed. Eight HIV subtypes including B(22 strains), B'(8 strains),C( 1 strain) ,CRF01_AE (38 strains) ,CRF02_AG (2 strains) ,CRF07_BC(9 strains) ,CRF08_BC(3 strains) and C/CRF01_AE recombinant like strain( 1 strain) were identified circulating in Beijing. Conclusion CRF01 _AE and subtype B were predominant in Beijing account for 45.2% and 26.2% and the surveillance of HIV gene variation should be paid more attention.