Immunogenicity of DNA vaccines encoding regulatory/accessory proteins derived from three different prevalent strains in China
10.3760/cma.j.issn.0254-5101.2011.02.013
- VernacularTitle:三个不同亚型HIV-1调节/辅助基因DNA疫苗的构建和免疫原性研究
- Author:
Xiaoyan XIE
;
Yanmin WAN
;
Bin LI
;
Jijing SHI
;
Chao QIU
;
Zhaoqi LIU
;
Jianqing XU
;
Huanxiang ZHANG
- Publication Type:Journal Article
- Keywords:
HIV/AIDS;
Vaccine;
T cell;
Regulatory/accessory protein;
Subdominant epitope
- From:
Chinese Journal of Microbiology and Immunology
2011;31(2):157-161
- CountryChina
- Language:Chinese
-
Abstract:
0bjective To determine the immunogenicities of DNA vaccines expressing tat-rev-integrase(c-half)-vif-neffusion genes(TRIVN) derived from prevalent B', B'/C and AE recombinant subtypes of HIV-1 in China. Methods Two DNA vaccines were constructed by inserting the codon optimized tat-revintegrase(c-half)-vif-nef fusion genes derived from B' and B'/C subtype of HIV-1 into mammalian expression vector pSVI. 0. DNA vaccine containing tat-rev-integrase (c-half)-vif-nef fusion gene derived from HIV-1AE2f has been constructed previously. In vitro expression efficiencies of three DNA vaccines were determined by Western blot and their immunogenicities were compared by immunizing female BALB/c mice. IFN-γ ELISPOT assay was used to read out the specific T cell immunity. Results The constructed DNA vaccines were validated by restriction enzyme digestion and DNA sequencing. Western blot assay showed three constructed DNA vaccines could be expressed at a comparable level in vitro. After vaccination, AE-TRIVN mounted T cell immune responses at (948.0 ± 330.0) SFCs/106 splenocytes, followed by the mixed DNA vaccine[ (500.0 ± 155.0) SFCs/106 splenocytes ], RL-TRIVN r[ ( 195. 1 ± 44.0) SFCs/106 splenocytes ]and CN-TRIVN [ (89.5 ± 17.0) SFCs/106 splenocytes]. Interestingly, we observed that single DNA vaccination induced specific T cell responses predominantly targeting Integrase (C-half) and Vif, whereas the mixed DNA could significantly improve T cell responses against Nef. Conclusion AE-TRIVN was the most immunogenic among the three DNA vaccines and the mixed DNA vaccination could change the immunogenic hierarchy of T cell epitopes across the fusion genes vaccine.
